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J Infect Dis. 2011 Oct 1;204(7):1075-85. doi: 10.1093/infdis/jir515.

BCG vaccination induces different cytokine profiles following infant BCG vaccination in the UK and Malawi.

Author information

  • 1Department of Immunology and Infection, London School of Hygiene and Tropical Medicine, United Kingdom. maeve_lalor@yahoo.com

Abstract

BACKGROUND:

BCG vaccination of infants is thought to provide good protection in all settings. This study investigated whether Malawian infants made weaker responses across a cytokine panel after BCG vaccination, compared with UK infants.

METHODS:

Diluted whole-blood samples were cultured with Mycobacterium tuberculosis purified protein derivative for 6 days from BCG-vaccinated infants 3 months (n = 40 Malawi, 28 UK) and 12 months (n = 34 Malawi, 26 UK) after vaccination, and also from UK unvaccinated infants (n = 9 at 3 months, n = 10 at 12 months). Forty-two cytokines were measured in supernatants using a multiplex bead array assay. Principal component analysis was used to summarize the overall patterns in cytokine responses.

RESULTS:

We found differences in median responses in 27 of the 42 cytokines: 7 higher in the UK and 20 higher in Malawi. The cytokines with higher responses in the UK were all T helper 1 related. The cytokines with higher responses in Malawi included innate proinflammatory cytokines, regulatory cytokines, interleukin 17, T helper 2 cytokines, chemokines, and growth factors. Principal component analysis separated the BCG-vaccinated infants from Malawi from the UK vaccinated infants and from the unvaccinated infants.

CONCLUSIONS:

Malawian infants make cytokine responses following BCG vaccination, but the cytokine profile is different from that in the UK. The different biosignatures following BCG vaccination in the 2 settings may indicate variability in the protective efficacy of infant BCG vaccination.

PMID:
21881123
[PubMed - indexed for MEDLINE]
PMCID:
PMC3164434
Free PMC Article
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