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Anal Bioanal Chem. 2012 Jan;402(1):231-47. doi: 10.1007/s00216-011-5308-5. Epub 2011 Aug 31.

Hydrophilic interaction liquid chromatography (HILIC)--a powerful separation technique.

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  • 1Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland. bbusz@chem.uni.torun.pl

Abstract

Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. One interesting option may also be to use HILIC in orthogonal and/or two-dimensional separations. Bioapplications of HILIC systems are also presented.

PMID:
21879300
[PubMed - indexed for MEDLINE]
PMCID:
PMC3249561
Free PMC Article
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