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Genome Biol. 2011 Aug 29;12(8):R80. doi: 10.1186/gb-2011-12-8-r80.

Single-cell copy number variation detection.

Author information

  • 1Department of Electrical Engineering, Esat-SCD, Katholieke Universiteit Leuven, Kasteelpark Arenberg 10, Leuven 3001, Belgium.

Abstract

Detection of chromosomal aberrations from a single cell by array comparative genomic hybridization (single-cell array CGH), instead of from a population of cells, is an emerging technique. However, such detection is challenging because of the genome artifacts and the DNA amplification process inherent to the single cell approach. Current normalization algorithms result in inaccurate aberration detection for single-cell data. We propose a normalization method based on channel, genome composition and recurrent genome artifact corrections. We demonstrate that the proposed channel clone normalization significantly improves the copy number variation detection in both simulated and real single-cell array CGH data.

PMID:
21854607
[PubMed - indexed for MEDLINE]
PMCID:
PMC3245619
Free PMC Article

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