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Genome Biol. 2011 Aug 11;12(8):R73. doi: 10.1186/gb-2011-12-8-r73.

Hybrid selection for sequencing pathogen genomes from clinical samples.

Author information

  • 1Genome Sequencing and Analysis Program, Broad Institute, Cambridge, MA 02142, USA.

Abstract

We have adapted a solution hybrid selection protocol to enrich pathogen DNA in clinical samples dominated by human genetic material. Using mock mixtures of human and Plasmodium falciparum malaria parasite DNA as well as clinical samples from infected patients, we demonstrate an average of approximately 40-fold enrichment of parasite DNA after hybrid selection. This approach will enable efficient genome sequencing of pathogens from clinical samples, as well as sequencing of endosymbiotic organisms such as Wolbachia that live inside diverse metazoan phyla.

PMID:
21835008
[PubMed - indexed for MEDLINE]
PMCID:
PMC3245613
Free PMC Article

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