Abstract
We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.
Publication types
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Clinical Laboratory Techniques / methods*
-
Cryptococcosis / diagnosis
-
Cryptococcosis / microbiology*
-
Cryptococcus gattii / classification*
-
Cryptococcus gattii / genetics
-
Cryptococcus gattii / isolation & purification
-
Cryptococcus neoformans / classification*
-
Cryptococcus neoformans / genetics
-
Cryptococcus neoformans / isolation & purification
-
DNA, Fungal / chemistry
-
DNA, Fungal / genetics*
-
Humans
-
Mycology / methods*
-
Sensitivity and Specificity
-
Temperature
-
Time Factors
-
Transition Temperature*