J Bioenerg Biomembr. 2011 Oct;43(5):447-55. Epub 2011 Aug 9.

Structural elements of the C-terminal domain of subunit E (E₁₃₃₋₂₂₂) from the Saccharomyces cerevisiae V₁V₀ ATPase determined by solution NMR spectroscopy.

Source

School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Republic of Singapore.

Abstract

Subunit E of the vacuolar ATPase (V-ATPase) contains an N-terminal extended α helix (Rishikesan et al. J Bioenerg Biomembr 43:187-193, 2011) and a globular C-terminal part that is predicted to consist of a mixture of α-helices and β-sheets (Grüber et al. Biochem Biophys Res Comm 298:383-391, 2002). Here we describe the production, purification and 2D structure of the C-terminal segment E₁₃₃₋₂₂₂ of subunit E from Saccharamyces cerevisiae V-ATPase in solution based on the secondary structure calculation from NMR spectroscopy studies. E₁₃₃₋₂₂₂ consists of four β-strands, formed by the amino acids from K136-V139, E170-V173, G186-V189, D195-E198 and two α-helices, composed of the residues from R144-A164 and T202-I218. The sheets and helices are arranged as β1:α1:β2:β3:β4:α2, which are connected by flexible loop regions. These new structural details of subunit E are discussed in the light of the structural arrangements of this subunit inside the V₁- and V₁V₀ ATPase.

PMID:: 21826517; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Saccharomyces Genome Database

Supplemental Content

Save items

Related citations in PubMed

NMR solution structure of subunit E (fragment E(1-69)) of the Saccharomyces cerevisiae V (1)V (O) ATPase. [J Bioenerg Biomembr. 2011]
NMR solution structure of subunit E (fragment E(1-69)) of the Saccharomyces cerevisiae V (1)V (O) ATPase.
Rishikesan S, Thaker YR, Grüber G. J Bioenerg Biomembr. 2011 Apr; 43(2):187-93. Epub 2011 Mar 12.
The NMR solution structure of subunit G (G(61)(-)(101)) of the eukaryotic V1VO ATPase from Saccharomyces cerevisiae. [Biochim Biophys Acta. 2010]
The NMR solution structure of subunit G (G(61)(-)(101)) of the eukaryotic V1VO ATPase from Saccharomyces cerevisiae.
Rishikesan S, Manimekalai MS, Grüber G. Biochim Biophys Acta. 2010 Oct; 1798(10):1961-8. Epub 2010 Jun 27.
Assembly of subunit d (Vma6p) and G (Vma10p) and the NMR solution structure of subunit G (G(1-59)) of the Saccharomyces cerevisiae V(1)V(O) ATPase. [Biochim Biophys Acta. 2009]
Assembly of subunit d (Vma6p) and G (Vma10p) and the NMR solution structure of subunit G (G(1-59)) of the Saccharomyces cerevisiae V(1)V(O) ATPase.
Rishikesan S, Gayen S, Thaker YR, Vivekanandan S, Manimekalai MS, Yau YH, Shochat SG, Grüber G. Biochim Biophys Acta. 2009 Apr; 1787(4):242-51. Epub 2009 Jan 22.
Review Structural features and nucleotide-binding capability of the C subunit are integral to the regulation of the eukaryotic V1Vo ATPases. [Biochem Soc Trans. 2005]
Review Structural features and nucleotide-binding capability of the C subunit are integral to the regulation of the eukaryotic V1Vo ATPases.
Grüber G. Biochem Soc Trans. 2005 Aug; 33(Pt 4):883-5.
Review Structure and function of the yeast vacuolar membrane proton ATPase. [J Bioenerg Biomembr. 1989]
Review Structure and function of the yeast vacuolar membrane proton ATPase.
Anraku Y, Umemoto N, Hirata R, Wada Y. J Bioenerg Biomembr. 1989 Oct; 21(5):589-603.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.

Recent activity

Clear Turn Off Turn On

Structural elements of the C-terminal domain of subunit E (E₁₃₃₋₂₂₂) from the Sa...
Structural elements of the C-terminal domain of subunit E (E₁₃₃₋₂₂₂) from the Saccharomyces cerevisiae V₁V₀ ATPase determined by solution NMR spectroscopy.
J Bioenerg Biomembr. 2011 Oct ;43(5):447-55. Epub 2011 Aug 9 .

PubMed
Deep-vein thrombosis after fracture of the pelvis: assessment with serial duplex...
Deep-vein thrombosis after fracture of the pelvis: assessment with serial duplex-ultrasound screening.
J Bone Joint Surg Am. 1990 Apr ;72(4):495-500.

PubMed
Distinct RNA degradation pathways and 3' extensions of yeast non-coding RNA spec...
Distinct RNA degradation pathways and 3' extensions of yeast non-coding RNA species.
Transcription. 2011 May;2(3):145-154.

PubMed
Philadelphia chromosome-negative chronic myelogenous leukemia and chronic myelom...
Philadelphia chromosome-negative chronic myelogenous leukemia and chronic myelomonocytic leukemia.
Hematol Oncol Clin North Am. 1990 Apr ;4(2):389-404.

PubMed
Rhabdomyolysis associated with the use of a mislabeled "acai berry" dietary supp...
Rhabdomyolysis associated with the use of a mislabeled "acai berry" dietary supplement.
Am J Med Sci. 2011 Dec ;342(6):535-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: