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PLoS One. 2011;6(7):e22678. doi: 10.1371/journal.pone.0022678. Epub 2011 Jul 26.

Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.

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  • 1Laboratory of Nanoscale Biology, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Abstract

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.

PMID:
21818365
[PubMed - indexed for MEDLINE]
PMCID:
PMC3144238
Free PMC Article

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