Cell. 2011 Aug 5;146(3):372-83. doi: 10.1016/j.cell.2011.07.003.

Spo11-accessory proteins link double-strand break sites to the chromosome axis in early meiotic recombination.

Panizza S, Mendoza MA, Berlinger M, Huang L, Nicolas A, Shirahige K, Klein F.

Source

Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 1, A-1030 Vienna, Austria.

Abstract

Meiotic recombination between homologous chromosomes initiates via programmed DNA double-strand breaks (DSBs), generated by complexes comprising Spo11 transesterase plus accessory proteins. DSBs arise concomitantly with the development of axial chromosome structures, where the coalescence of axis sites produces linear arrays of chromatin loops. Recombining DNA sequences map to loops, but are ultimately tethered to the underlying axis. How and when such tethering occurs is currently unclear. Using ChIPchip in yeast, we show that Spo11-accessory proteins Rec114, Mer2, and Mei4 stably interact with chromosome axis sequences, upon phosphorylation of Mer2 by S phase Cdk. This axis tethering requires meiotic axis components (Red1/Hop1) and is modulated in a domain-specific fashion by cohesin. Loss of Rec114, Mer2, and Mei4 binding correlates with loss of DSBs. Our results strongly suggest that hotspot sequences become tethered to axis sites by the DSB machinery prior to DSB formation.

PMID:: 21816273; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

Secondary Source ID

GEO/GSE29860

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Saccharomyces Genome Database

Supplemental Content

Save items

Related citations in PubMed

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae. [Chromosoma. 2007]
Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae.
Maleki S, Neale MJ, Arora C, Henderson KA, Keeney S. Chromosoma. 2007 Oct; 116(5):471-86. Epub 2007 Jun 9.
Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes. [Mol Cell. 2013]
Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes.
Sommermeyer V, Béneut C, Chaplais E, Serrentino ME, Borde V. Mol Cell. 2013 Jan 10; 49(1):43-54. Epub 2012 Dec 13.
The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination. [Science. 2013]
The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination.
Acquaviva L, Székvölgyi L, Dichtl B, Dichtl BS, de La Roche Saint André C, Nicolas A, Géli V. Science. 2013 Jan 11; 339(6116):215-8. Epub 2012 Nov 15.
Review Modulating and targeting meiotic double-strand breaks in Saccharomyces cerevisiae. [Methods Mol Biol. 2009]
Review Modulating and targeting meiotic double-strand breaks in Saccharomyces cerevisiae.
Nicolas A. Methods Mol Biol. 2009; 557:27-33.
Review The multiple roles of the Mre11 complex for meiotic recombination. [Chromosome Res. 2007]
Review The multiple roles of the Mre11 complex for meiotic recombination.
Borde V. Chromosome Res. 2007; 15(5):551-63.

See reviews... See all...

Cited by 12 PubMed Central articles

DNA double-strand break repair as determinant of cellular radiosensitivity to killing and target in radiation therapy. [Front Oncol. 2013]
DNA double-strand break repair as determinant of cellular radiosensitivity to killing and target in radiation therapy.
Mladenov E, Magin S, Soni A, Iliakis G. Front Oncol. 2013; 3:113. Epub 2013 May 10.
Differential Association of the Conserved SUMO Ligase Zip3 with Meiotic Double-Strand Break Sites Reveals Regional Variations in the Outcome of Meiotic Recombination. [PLoS Genet. 2013]
Differential Association of the Conserved SUMO Ligase Zip3 with Meiotic Double-Strand Break Sites Reveals Regional Variations in the Outcome of Meiotic Recombination.
Serrentino ME, Chaplais E, Sommermeyer V, Borde V. PLoS Genet. 2013 Apr; 9(4):e1003416. Epub 2013 Apr 4.
Dot1-dependent histone H3K79 methylation promotes activation of the Mek1 meiotic checkpoint effector kinase by regulating the Hop1 adaptor. [PLoS Genet. 2013]
Dot1-dependent histone H3K79 methylation promotes activation of the Mek1 meiotic checkpoint effector kinase by regulating the Hop1 adaptor.
Ontoso D, Acosta I, van Leeuwen F, Freire R, San-Segundo PA. PLoS Genet. 2013 Jan; 9(1):e1003262. Epub 2013 Jan 31.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
GEO DataSets
Gene expression and molecular abundance data reported in the current articles that are also included in the curated Gene Expression Omnibus (GEO) DataSets.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Related Project
Related Projects
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Spo11-accessory proteins link double-strand break sites to the chromosome axis i...
Spo11-accessory proteins link double-strand break sites to the chromosome axis in early meiotic recombination.
Cell. 2011 Aug 5 ;146(3):372-83. doi: 10.1016/j.cell.2011.07.003.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: