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Zhongguo Zhong Yao Za Zhi. 2011 Apr;36(8):1071-4.

[Screen anti-influenza virus serum marker of Lonicera japonica by proteomics technology].

[Article in Chinese]

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  • 1Shandong Academy of Traditional Chinese Medicine, Jinan 250014, China.

Abstract

OBJECTIVE:

The candidate anti-influenza virus serum marker of Lonicera japonica was searched by comparing the serum protein discrepancy of experimental groups. It will provide the basis for setting forth the action and mechanism of anti-influenza virus.

METHOD:

Two-dimensional electrophoresis (2-DE) was used to separate different experimental groups and the differences in serum protein was compared. The significantly expressed protein spots was selected for mass spectrum identification and bioinformatics analysis.

RESULT:

Fifteen identified points of protein have more typical differences between experimental groups. The structures were identified by MALDI-TOF-MS, and 12 functional protein component ownership were retrieved.

CONCLUSION:

There was significant difference between the serum protein of experimental groups Proteomic methods can be used to select and identify the serum marker. It is expected to clarify the mechanism of anti-influenza virus of L. japonica.

PMID:
21809588
[PubMed - indexed for MEDLINE]
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