Source
Department of Pharmacology, JIPMER, Pondicherry, India.
Abstract
OBJECTIVE:
To determine the in vitro free radical scavenging property and in vivo diuretic effect of Triglize™ , a marketed polyherbal formulation in experimental models.
MATERIALS AND METHODS:
The aqueous extract of polyherbal formulation (PHF) triglize was used for the experiment. The free radical scavenging property and antioxidant effect of PHF were studied by LPS-induced free radicals in rat macrophages cells and DPPH (2, 2-Diphenyl-1-Picrylhydrazyl) methods, respectively. The diuretic effect of a PHF was studied with Lipschitz model using male Wistar rats.
RESULTS:
PHF significantly inhibited lipopolysaccharide -induced free radicals in rat macrophages and it showed moderate antioxidant potential in DPPH model. Polyherbal formulation at 50, 200 and 400 mg/ kg significantly increased potassium excretion in urine at 0-5 h and 5-24 h. The diuretic effect of PHF was as similar as furosemide.
CONCLUSION:
The PHF has significant diuretic effect and free radical scavenging properties.