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J Biomed Opt. 2011 Jul;16(7):076002. doi: 10.1117/1.3594785.

Photoacoustic microscopy of microvascular responses to cortical electrical stimulation.

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  • 1Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130, USA.

Abstract

Advances in the functional imaging of cortical hemodynamics have greatly facilitated the understanding of neurovascular coupling. In this study, label-free optical-resolution photoacoustic microscopy (OR-PAM) was used to monitor microvascular responses to direct electrical stimulations of the mouse somatosensory cortex through a cranial opening. The responses appeared in two forms: vasoconstriction and vasodilatation. The transition between these two forms of response was observed in single vessels by varying the stimulation intensity. Marked correlation was found between the current-dependent responses of two daughter vessels bifurcating from the same parent vessel. Statistical analysis of twenty-seven vessels from three different animals further characterized the spatial-temporal features and the current dependence of the microvascular response. Our results demonstrate that OR-PAM is a valuable tool to study neurovascular coupling at the microscopic level.

PMID:
21806263
[PubMed - indexed for MEDLINE]
PMCID:
PMC3144972
Free PMC Article
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