Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes

Appl Environ Microbiol. 2011 Sep;77(18):6699-702. doi: 10.1128/AEM.00370-11. Epub 2011 Jul 29.

Abstract

Specificity analysis for stx or Stx subtypes in Escherichia coli showed that the PCR assays we tested did not detect stx(1d) and stx(2f), and some also missed stx(2b) and stx(2g). Most of the serological assays examined did not detect Stx2c, Stx2e, Stx2f, and Stx2g, and some strain-to-strain variation in reactivity was observed for Stx2b.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Escherichia coli / pathogenicity*
  • Genotype
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Serologic Tests / methods
  • Serotyping
  • Shiga Toxin / analysis*
  • Shiga Toxin / classification
  • Shiga Toxin / genetics
  • Shiga Toxin / immunology

Substances

  • Shiga Toxin