Flowchart illustrating the proteogenomics analysis steps.

Mapping of mass spectrometry-derived peptide data onto the VectorBase genome browser. The unique peptides identified by mass spectrometry (rectangle bars), which mapped to the known exons of the gene encoding salivary gland secreted protein 4 (SGS4) (AGAP009917-RA). The peptides identified in this study can be viewed as separate tracks on the VectorBase genome browser using the URL http://funcgen.vector base.org/gdav/das as DAS server and “JHU_Ag_v2” as the data source. The JHU_Ag_v2 track shows peptide data as JHU_Ag_xxxx, where JHU and Ag stand for Johns Hopkins University and An. gambiae, respectively; and “xxxx” denotes the serial number of the peptide. The MS/MS spectra of two representative peptides LESMLEYSDVQIDR (JHU_Ag_24279) and TVDIFVANMITFR (JHU_Ag_41147) are shown.

Overview of mass spectrometry data used for genome annotation. (A) An estimation of the mass error of peptides in parts per million identified from mass spectrometric analysis of An. gambiae. (B) Chromosomal distribution of peptides identified by mass spectrometry. The number of peptides identified from each chromosome roughly parallels the estimated number of known and novel protein-coding genes in An. gambiae.

(A) N-terminal extension of AGAP011939 using peptides mapping to an upstream intergenic region. Twenty peptides were mapped to an intergenic region upstream of the gene AGAP011939. SNAP predicts a longer gene model that is supported by novel peptides identified upstream of this gene. (B) Identification of a novel protein-coding gene using peptides mapping to an intergenic region. Sixteen peptides were mapped to an intergenic region on chromosome 3R, where the intron of a VectorBase gene model AGAP009515-RA was annotated on the opposite strand. The presence of a novel gene in this region is also indicated by the SNAP prediction program. (C) Correction of a gene structure using peptide mapping to an intron of an annotated gene. Fifteen peptides were identified in the intronic region of the gene AGAP008769. These peptides support two different gene models predicted by SNAP. (D) Identification of peptides translated in a different frame from the annotated protein sequence. Three GSSPs mapped within the coordinates of the sixth exon of the AGAP000622 gene that were not present in the predicted protein product of the gene. However, these peptides were present in the protein product of SNAP prediction and NCBI RefSeq annotation. (E) Identification of a novel protein-coding region using peptides mapping to the UTR of a gene. Five GSSPs mapped to the 3′-UTR region of the AGAP009974 gene. The SNAP prediction model for this genomic region supports a C-terminal extension of the protein encoded by the AGAP009974 gene. (F) Identification of a novel splice form. The peptide, IIEDSDYVAVLFYKPECK, was identified in the MS/MS ion search against the novel splice junction database of hypothetical splice isoforms. This novel splicing event, which occurred between exons 3 and 5 of the AGAP006452-RA gene, is also observed in Culex quinquefasciatus.

Validation of mass spectrometry-derived data using RT-PCR and cDNA sequencing. RT-PCR products were sequenced on both strands, and the resulting sequences were submitted to GenBank. GenBank accession numbers are indicated above each lane. (A) RT-PCR validation of 35 novel genes. (B) RT-PCR validation of 70 gene models that led to the correction of existing VectorBase gene annotations in genebuild AgambP3.4. The genes that belong to novel categories with respect to genebuild AgambP3.6 are marked with an asterisk (*).