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Genome Res. 2011 Oct;21(10):1650-8. doi: 10.1101/gr.121145.111. Epub 2011 Jul 27.

Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1.

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  • 1Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Abstract

Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.

PMID:
21795385
[PubMed - indexed for MEDLINE]
PMCID:
PMC3202282
Free PMC Article

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