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    J Nucleic Acids. 2011;2011:756905. doi: 10.4061/2011/756905. Epub 2011 May 18.

    Validation of a diagnostic microarray for human papillomavirus: coverage of 102 genotypes.

    Arron ST, Skewes-Cox P, Do PH, Dybbro E, Da Costa M, Palefsky JM, Derisi JL.

    Source

    Department of Dermatology, University of California San Francisco, San Francisco, CA 94143, USA.

    Abstract

    Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values.

    PMID:
    21785699
    [PubMed]
    PMCID:
    PMC3139178
    Free PMC Article

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