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J Nucleic Acids. 2011;2011:870726. doi: 10.4061/2011/870726. Epub 2011 Jun 9.

Distribution of 5-hydroxymethylcytosine in different human tissues.

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  • 1R&D Division, Epigentek Group Inc., 110 Bi County Boulevard, Suite 122, Farmingdale, NY 11735, USA.

Abstract

5-hydroxymethylcytosine (5-hmC) is a modified form of cytosine recently found in mammalians and is believed, like 5-methylcytosine, to also play an important role in switching genes on and off. By utilizing a newly developed 5-hmC immunoassay, we determined the abundance of 5-hmC in human tissues and compared 5-hmC states in normal colorectal tissue and cancerous colorectal tissue. Significant differences of 5-hmC content in different tissues were observed. The percentage of 5-hmC measured is high in brain, liver, kidney and colorectal tissues (0.40-0.65%), while it is relatively low in lung (0.18%) and very low in heart, breast, and placenta (0.05-0.06%). Abundance of 5-hmC in the cancerous colorectal tissues was significantly reduced (0.02-0.06%) compared to that in normal colorectal tissues (0.46-0.57%). Our results showed for the first time that 5-hmC distribution is tissue dependent in human tissues and its abundance could be changed in the diseased states such as colorectal cancer.

PMID:
21772996
[PubMed]
PMCID:
PMC3136188
Free PMC Article

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