Blood. 2011 Sep 29;118(13):e74-81. Epub 2011 Jul 18.

A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax.

Russell B, Suwanarusk R, Borlon C, Costa FT, Chu CS, Rijken MJ, Sriprawat K, Warter L, Koh EG, Malleret B, Colin Y, Bertrand O, Adams JH, D'Alessandro U, Snounou G, Nosten F, Rénia L.

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Singapore Immunology Network, Biopolis, Agency for Science Technology and Research, Singapore. bruce_russell@immunol.a-star.edu.sg

Abstract

Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand.

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Blood. 2011 Sep 29;118(13):3454-5.

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A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax.
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A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax.

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