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Int J Biol Macromol. 2011 Nov 1;49(4):700-6. doi: 10.1016/j.ijbiomac.2011.07.002. Epub 2011 Jul 13.

Comparative analysis of gelatin scaffolds crosslinked by genipin and silane coupling agent.

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  • 1Department of Mechanics, Politecnico di Torino, Turin, Italy.

Abstract

Scaffolds based on gelatin (G) are considered promising for tissue engineering, able to mimic the natural extracellular matrix. G drawback is its poor structural consistency in wet conditions. Therefore, crosslinking is necessary to fabricate stable G scaffolds. In this work, a comparative study between the performance of two different crosslinkers, genipin (GP) and γ-glycidoxypropyltrimethoxysilane (GPTMS), is presented. Flat membranes by solvent casting and porous crosslinked scaffolds by freeze-drying were prepared. Infrared spectroscopy and thermal analysis were applied to confirm G chain crosslinking. Moreover, GP and GPTMS increased the stability of G in aqueous media and improved the mechanical properties. Crosslinking reduced the wettability, especially in the case of G_GPTMS samples, due to the introduction of hydrophobic siloxane chains. Both G_GP and G_GPTMS scaffolds supported MG-63 osteoblast-like cell adhesion and proliferation.

Copyright © 2011 Elsevier B.V. All rights reserved.

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