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Nat Methods. 2011 Jul 17;8(9):761-3. doi: 10.1038/nmeth.1650.

Bayesian community-wide culture-independent microbial source tracking.

Author information

  • 1Department of Computer Science, University of Colorado, Boulder, Colorado, USA.

Abstract

Contamination is a critical issue in high-throughput metagenomic studies, yet progress toward a comprehensive solution has been limited. We present SourceTracker, a Bayesian approach to estimate the proportion of contaminants in a given community that come from possible source environments. We applied SourceTracker to microbial surveys from neonatal intensive care units (NICUs), offices and molecular biology laboratories, and provide a database of known contaminants for future testing.

PMID:
21765408
[PubMed - indexed for MEDLINE]
PMCID:
PMC3791591
Free PMC Article

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