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Stem Cell Res. 2011 Sep;7(2):154-62. doi: 10.1016/j.scr.2011.04.005. Epub 2011 May 16.

Human feeder cell line for derivation and culture of hESc/hiPSc.

Author information

  • 1North West Embryonic Stem Cell Centre, Faculty of Life Sciences, Core Technology Facility, University of Manchester, 46 Grafton Street, Manchester, M13 9NT, UK. t.mckay@qmul.ac.uk

Abstract

We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.

Copyright © 2011 Elsevier B.V. All rights reserved.

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