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Clin Biochem. 2011 Oct;44(14-15):1241-6. doi: 10.1016/j.clinbiochem.2011.06.983. Epub 2011 Jul 6.

Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.

Author information

  • 1Department of Molecular Cell Biology Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands. P.Corstjens@LUMC.NL

Abstract

OBJECTIVE:

The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.

DESIGN AND METHODS:

A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.

RESULTS:

Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.

CONCLUSIONS:

The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.

Copyright © 2011 Elsevier B.V. All rights reserved.

PMID:
21763300
[PubMed - indexed for MEDLINE]
PMCID:
PMC3177995
Free PMC Article
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