Curr Opin Struct Biol. 2011 Aug;21(4):460-6. Epub 2011 Jul 15.

Computational design of membrane proteins.

Source

University of Wisconsin-Madison, Department of Biochemistry, 433 Babcock Dr., Madison, WI 53706, USA. senes@wisc.edu

Abstract

This article reviews the recent successes of computational protein design techniques applied to integral membrane proteins. This emerging area is still handicapped by significant difficulties in the experimental characterization of the stability and structure of the designed proteins. Nevertheless, by focusing on oligomeric complexes of single-span transmembrane (TM) peptides with detectable activity, the computational design of membrane proteins has already produced very exciting results. The 'take-home message' is that optimization of van der Waals packing and hydrogen bonding (both 'canonical' and weak Cα-H⋯O bonds) can produce functional structures of remarkable stability and specificity in the membrane.

PMID:: 21763125; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Review

MeSH Terms

Substances

Membrane Proteins

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Related citations

Review Modulating membrane protein stability and association by design. [Curr Opin Struct Biol. 2007]
Review Modulating membrane protein stability and association by design.
Barth P. Curr Opin Struct Biol. 2007 Aug; 17(4):460-6. Epub 2007 Sep 17.
Structural determinants of transmembrane helical proteins. [Structure. 2009]
Structural determinants of transmembrane helical proteins.
Harrington SE, Ben-Tal N. Structure. 2009 Aug 12; 17(8):1092-103.
De novo protein design. I. In search of stability and specificity. [J Mol Biol. 1999]
De novo protein design. I. In search of stability and specificity.
Koehl P, Levitt M. J Mol Biol. 1999 Nov 12; 293(5):1161-81.
Computer modeling of polyleucine-based coiled coil dimers in a realistic membrane environment: insight into helix-helix interactions in membrane proteins. [Biochemistry. 2004]
Computer modeling of polyleucine-based coiled coil dimers in a realistic membrane environment: insight into helix-helix interactions in membrane proteins.
Ash WL, Stockner T, MacCallum JL, Tieleman DP. Biochemistry. 2004 Jul 20; 43(28):9050-60.
Review Challenges in the computational design of proteins. [J R Soc Interface. 2009]
Review Challenges in the computational design of proteins.
Suárez M, Jaramillo A. J R Soc Interface. 2009 Aug 6; 6 Suppl 4:S477-91. Epub 2009 Mar 11.

See reviews... See all...

Recent activity

Clear Turn Off Turn On

Computational design of membrane proteins.
Computational design of membrane proteins.
Curr Opin Struct Biol. 2011 Aug ;21(4):460-6. Epub 2011 Jul 15 .

PubMed
The β subunit gate loop is required for RNA polymerase modification by RfaH and ...
The β subunit gate loop is required for RNA polymerase modification by RfaH and NusG.
Mol Cell. 2011 Jul 22 ;43(2):253-62.

PubMed
Role of the RNA polymerase trigger loop in catalysis and pausing.
Role of the RNA polymerase trigger loop in catalysis and pausing.
Nat Struct Mol Biol. 2010 Jan ;17(1):99-104. Epub 2009 Dec 6 .

PubMed
RNA transcript 3'-proximal sequence affects translocation bias of RNA polymerase...
RNA transcript 3'-proximal sequence affects translocation bias of RNA polymerase.
Biochemistry. 2011 Aug 16 ;50(32):7002-14. Epub 2011 Jul 21 .

PubMed
The NusA N-terminal domain is necessary and sufficient for enhancement of transc...
The NusA N-terminal domain is necessary and sufficient for enhancement of transcriptional pausing via interaction with the RNA exit channel of RNA polymerase.
J Mol Biol. 2010 Sep 3 ;401(5):708-25. Epub 2010 Jun 25 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to:

Computational design of membrane proteins.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Related citations

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information