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Tissue Antigens. 2011 Oct;78(4):275-80. doi: 10.1111/j.1399-0039.2011.01737.x. Epub 2011 Jul 18.

Multiplexed, ligation-dependent probe amplification for rapid and inexpensive HLA-DQB1 allelotyping.

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  • 1Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, Berkeley, CA, USA. kipp@berkeley.edu

Abstract

Many effective options exist to accurately type DNA for human leukocyte antigen (HLA) alleles. However, most of the existing methods are excessively costly in terms of overall monetary costs, DNA requirements, and proprietary software. We present a novel assay capable of resolving heterozygous HLA-DQB1 allelotypes at two digits, with even greater specificity for the HLA-DQB1*06 allele family, by using the multiplexed ligation-dependent probe amplification technology. This assay provides more specific allele data than genome-wide analysis and is more affordable than sequencing, making it a useful intermediate for researchers seeking to accurately allelotype human DNA samples.

© 2011 John Wiley & Sons A/S.

PMID:
21762399
[PubMed - indexed for MEDLINE]
PMCID:
PMC3431962
Free PMC Article

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