Proteomics. 2011 Aug;11(16):3415-9. doi: 10.1002/pmic.201000587. Epub 2011 Jul 13.

Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.

Corrigan L, Jefferies C, Clive Lee T, Daly J.

Source

Division of Biology, Anatomy Department, Royal College of Surgeons in Ireland, Dublin, Ireland. lauracorrigan@rcsi.ie

Abstract

Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.

PMID:: 21751350; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

Immunoaffinity separation of plasma proteins by IgY microbeads: meeting the needs of proteomic sample preparation and analysis. [Proteomics. 2005]
Immunoaffinity separation of plasma proteins by IgY microbeads: meeting the needs of proteomic sample preparation and analysis.
Huang L, Harvie G, Feitelson JS, Gramatikoff K, Herold DA, Allen DL, Amunngama R, Hagler RA, Pisano MR, Zhang WW, et al. Proteomics. 2005 Aug; 5(13):3314-28.
Comparison of protein enrichment strategies for proteome analysis of plasma. [Proteomics. 2010]
Comparison of protein enrichment strategies for proteome analysis of plasma.
Bandow JE. Proteomics. 2010 Apr; 10(7):1416-25.
High-abundance protein depletion: comparison of methods for human plasma biomarker discovery. [Electrophoresis. 2010]
High-abundance protein depletion: comparison of methods for human plasma biomarker discovery.
Polaskova V, Kapur A, Khan A, Molloy MP, Baker MS. Electrophoresis. 2010 Jan; 31(3):471-82.
Review Human blood plasma preparation for two-dimensional gel electrophoresis. [J Chromatogr B Analyt Technol ...]
Review Human blood plasma preparation for two-dimensional gel electrophoresis.
Kim MR, Kim CW. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15; 849(1-2):203-10. Epub 2006 Dec 15.
Review Current two-dimensional electrophoresis technology for proteomics. [Proteomics. 2004]
Review Current two-dimensional electrophoresis technology for proteomics.
Görg A, Weiss W, Dunn MJ. Proteomics. 2004 Dec; 4(12):3665-85.

See reviews... See all...

Recent activity

Clear Turn Off Turn On

Evaluation and optimization of IgY spin column technology in the depletion of ab...
Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.
Proteomics. 2011 Aug ;11(16):3415-9. doi: 10.1002/pmic.201000587. Epub 2011 Jul 13 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.

Source

Abstract

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information