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Angew Chem Int Ed Engl. 2011 Aug 22;50(35):8077-81. doi: 10.1002/anie.201102646. Epub 2011 Jul 12.

Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison.

Author information

  • 1Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Abstract

Capturing the right ligand at the right spot: a well-balanced system for non-natural amino acid mutagenesis allows the ligand binding sites of a class II G-protein coupled receptor to be mapped and distinct binding domains to be identified for different ligands in the native environment of mammalian cells.

Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PMID:
21751313
[PubMed - indexed for MEDLINE]
PMCID:
PMC3262126
Free PMC Article
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