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Inflamm Res. 2011 Oct;60(10):991-5. doi: 10.1007/s00011-011-0361-2. Epub 2011 Jul 13.

Inflammation inhibits GPR81 expression in adipose tissue.

Author information

  • 1Metabolism Section (111F), Department of Veterans Affairs Medical Center, University of California San Francisco, 4150 Clement Street, San Francisco, CA 94121, USA. kenneth.feingold@ucsf.edu

Abstract

OBJECTIVE AND DESIGN:

The aim of this study was to examine the expression of G protein-coupled receptor 81 (GPR81) in mouse adipose tissue in response to inflammatory stimuli. GPR81 is activated by lactate resulting in the inhibition of lipolysis.

MATERIALS AND TREATMENT:

Mice were injected with saline, lipopolysaccharide (LPS), zymosan, or turpentine, N = 5 per group. 3T3-L1 adipocytes were treated with tumor necrosis factor alpha, interleukin (IL)-l beta, IL-6, or interferon gamma.

METHODS:

GPR81 expression levels were measured by real-time PCR and statistical significance was determined by Student's t test.

RESULTS:

LPS resulted in a marked decrease in GPR81 mRNA level in mouse adipose tissue in C57BL/6 and OuJ mice, an effect that was not observed in HeJ mice, which have a mutation in TLR4. Zymosan and turpentine also decreased adipose tissue GPR81 expression. Cytokine treatment of 3T3-L1 adipocytes had no effect on GPR81 expression. GPR81 expression was decreased in ob/ob mice, an animal model of type 2 diabetes that is characterized by inflammation.

CONCLUSION:

Inflammation decreases the expression of GPR81 in adipose tissue.

PMID:
21751047
[PubMed - indexed for MEDLINE]
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