Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy

Doori Oh; Seungah Lee; Seong Ho Kang

doi:10.1039/c1cc12911b

Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy

Chem Commun (Camb). 2011 Aug 28;47(32):9137-9. doi: 10.1039/c1cc12911b. Epub 2011 Jul 13.

Authors

Doori Oh¹, Seungah Lee, Seong Ho Kang

Affiliation

¹ Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea.

PMID: 21750823
DOI: 10.1039/c1cc12911b

Abstract

In a free solution of 10.0 mM Gly-Gly (pH 8.2), the flow directions of native-DNA and DNA molecules intercalated with the fluorescent dye YOYO-1 were reversed in the microchip channel. These results clearly showed that in a confined space and under specific environmental conditions, the fluorescent dye modified the original properties and behavior of the native-DNA molecule.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Benzoxazoles / chemistry*
DNA / chemistry*
Equipment Design
Fluorescent Dyes / chemistry*
Glycylglycine / chemistry
Intercalating Agents / chemistry*
Microscopy, Interference / instrumentation*
Oligonucleotide Array Sequence Analysis*
Quinolinium Compounds / chemistry*

Substances

Benzoxazoles
Fluorescent Dyes
Intercalating Agents
Quinolinium Compounds
Glycylglycine
1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium
DNA