Human cadaver specimen. Comparison between corresponding OCT (a), μOCT (b), and histology images (c, Hematoxylin and Eosin) of a calcium plate (Ca) within the coronary artery wall. Scale bar, 200 μm.

(a) Three-dimensional rendering of the swine coronary artery ex vivo, demonstrating a pattern of raised cells that are consistent with endothelial “pavementing”. (b–h) Human cadaver specimens. (b) Multiple cells that are likely leukocytes (arrows) are seen adhering to the luminal surface in this μOCT image of a coronary plaque. Two different cell morphologies can be observed, one smaller cell with scant cytoplasm, consistent with a lymphocyte (yellow arrow) and another, slightly larger cell with a highly scattering, abundant cytoplasm, suggestive of a monocyte (green arrow). (c) Cell with an indented, bean-shaped nucleus (green arrow) characteristic of a monocyte. (d) Cell with a multi-lobed nucleus, possibly a neutrophil (blue arrow), is attached to the endothelial surface. (e) Multiple leukocytes tethered to the endothelial surface by linear structures suggestive of pseudopodia (white arrows). (f) Cells with the morphology of monocytes (red arrows) are seen in this cross-section and inset to be transmigrating through the endothelium. (g) Structures consistent with fibrin (magenta arrow) are visible as linear strands bridging a gap in the coronary artery wall. (h) Thrombus (cyan arrow) that appears to contain fibrin, small (2–3 μm diameter) highly scattering structures likely to be platelets, and multiple, entrapped cells. Scale bars, 30 μm.

Human cadaver specimens. (a) Necrotic core (nc) fibroatheroma with highly scattering lipid-laden macrophages or foam cells (white arrows) infiltrating the cap, also seen in the corresponding histology (upper left inset). An intracellular region of low μOCT signal, which may represent the nucleus, can be observed within the cytoplasm of some foam cells (e.g. lower left inset, blue arrow). (b) Another lesion, visualized by μOCT and histology, contains highly scattering foam cells that are ellipsoidal (right insets). (c) Smooth muscle cells by μOCT appear as spindle-shaped cells (green arrow). Smooth muscle cells producing collagen have a high backscattering interior (right upper inset, yellow arrow) and a “halo” of low backscattering (right upper inset, white arrow). Matching histology (right lower inset) demonstrates that the high backscattering region represents the cell body, while the lower intensity halo corresponds to collagen matrix. (d) Large necrotic core (nc) fibroatheroma, demonstrating cholesterol crystals (cc), characterized by reflections from their top and bottom surfaces. (e) A thin crystal (red arrow) appears to be piercing the cap of another necrotic core (nc) plaque. Scale bars for all primary images, 100 μm. Scale bars for all insets, 30 μm.

Human cadaver specimens. (a) μOCT image from a coronary segment with an implanted BMS shows struts devoid of polymer, covered by neointima. (b) DES struts from another cadaver showing polymer (red dashed box and p, inset) overlying the strut reflections. (c) Tissue (yellow arrow) is interposed between the polymer and the stent strut and the polymer has fractured (white arrow). (d) Superficial leukocyte cluster (cyan arrow) and adjacent attached leukocytes overlying the site of the polymer fracture. (e) Apparent inflammation at the edge of a strut (green arrow). (f) Uncovered strut, completely devoid of overlying endothelium (red dashed box and inset). Scale bars for primary images, 100 μm. Scale bars for all insets, 30 μm.