Abstract
The hydroalcoholic extract obtained from the seeds of Casimiroa edulis cultivated in Sardinia (Italy) have been assayed on the two enzymatic-associated activities of the HIV-1 reverse transcriptase (RT), the RNA-dependent DNA polymerase (RDDP) and the ribonuclease H. In biochemical assays, the extract inhibited both activities in a dose-dependent manner, showing a 10-fold more potent inhibition of the HIV-1 RT RDDP activity. Furthermore, the extract was cytotoxic on K562 cell replication.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Casimiroa / chemistry*
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Cell Line, Tumor
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Enzyme Activation / drug effects
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HIV Reverse Transcriptase / metabolism*
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Humans
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Plant Extracts / chemistry*
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Plant Extracts / pharmacology*
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RNA-Directed DNA Polymerase / metabolism
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Reverse Transcriptase Inhibitors / chemistry*
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Reverse Transcriptase Inhibitors / pharmacology*
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Ribonuclease H / metabolism
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Seeds / chemistry*
Substances
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Plant Extracts
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Reverse Transcriptase Inhibitors
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reverse transcriptase, Human immunodeficiency virus 1
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HIV Reverse Transcriptase
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RNA-Directed DNA Polymerase
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Ribonuclease H