J Biol Chem. 1990 Nov 5;265(31):18902-6.

Binding of 3'-anthraniloyl-2'-deoxy-ATP to calmodulin-activated adenylate cyclase from Bordetella pertussis and Bacillus anthracis.

Sarfati RS, Kansal VK, Munier H, Glaser P, Gilles AM, Labruyère E, Mock M, Danchin A, Bârzu O.

Source

Unités de Chimie Organique, Institut Pasteur, Paris, France.

Abstract

3'-Anthraniloyl-2'-deoxyadenosine 5'-triphosphate (Ant-dATP), a fluorescent analogue of ATP, was tested as a probe for the nucleotide-binding site of calmodulin (CaM)-activated adenylate cyclases from Bordetella pertussis (BPCYA47) and Bacillus anthracis (BACYA62). Ant-dATP competitively inhibited both bacterial enzymes expressed in Escherichia coli (ki approximately 10 microM). Binding of the analogue to adenylate cyclase was monitored by equilibrium dialysis and by an increase in its fluorescence emission at 420 nm upon excitation at 330 nm. Whereas the fluorescence of Ant-dATP was little influenced by divalent cations, CaM, or adenylate cyclase alone, the Ca2+.CaM.cyclase complex increased up to 4 times the quantum yield of Ant-dATP. Binding of the analogue to the catalytic site of BPCYA47 and BACYA62 was specific as shown by its displacement with ATP or 3'-dATP. Our results substantiate the role of CaM in favoring substrate binding to CaM-activated enzymes.

PMID:: 2172237; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

HighWire Press - PDF

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy. [Eur J Biochem. 2004]
Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy.
Gallay J, Vincent M, Li de la Sierra IM, Munier-Lehmann H, Renouard M, Sakamoto H, Bârzu O, Gilles AM. Eur J Biochem. 2004 Feb; 271(4):821-33.
Functional consequences of single amino acid substitutions in calmodulin-activated adenylate cyclase of Bordetella pertussis. [EMBO J. 1991]
Functional consequences of single amino acid substitutions in calmodulin-activated adenylate cyclase of Bordetella pertussis.
Glaser P, Munier H, Gilles AM, Krin E, Porumb T, Bârzu O, Sarfati R, Pellecuer C, Danchin A. EMBO J. 1991 Jul; 10(7):1683-8.
Relationships between the calmodulin-dependent adenylate cyclases produced by Bacillus anthracis and Bordetella pertussis. [Biochem Biophys Res Commun. 1988]
Relationships between the calmodulin-dependent adenylate cyclases produced by Bacillus anthracis and Bordetella pertussis.
Robertson DL. Biochem Biophys Res Commun. 1988 Dec 30; 157(3):1027-32.
Review Calmodulin-activated bacterial adenylate cyclases as virulence factors. [Trends Microbiol. 1993]
Review Calmodulin-activated bacterial adenylate cyclases as virulence factors.
Mock M, Ullmann A. Trends Microbiol. 1993 Aug; 1(5):187-92.
Review Mechanisms of bacterial pathogenicity that involve production of calmodulin-sensitive adenylate cyclases. [Microbiol Rev. 1987]
Review Mechanisms of bacterial pathogenicity that involve production of calmodulin-sensitive adenylate cyclases.
Masure HR, Shattuck RL, Storm DR. Microbiol Rev. 1987 Mar; 51(1):60-5.

See reviews... See all...

Cited by 3 PubMed Central articles

Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin. [EMBO J. 2005]
Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin.
Guo Q, Shen Y, Lee YS, Gibbs CS, Mrksich M, Tang WJ. EMBO J. 2005 Sep 21; 24(18):3190-201. Epub 2005 Sep 1.
Physiological calcium concentrations regulate calmodulin binding and catalysis of adenylyl cyclase exotoxins. [EMBO J. 2002]
Physiological calcium concentrations regulate calmodulin binding and catalysis of adenylyl cyclase exotoxins.
Shen Y, Lee YS, Soelaiman S, Bergson P, Lu D, Chen A, Beckingham K, Grabarek Z, Mrksich M, Tang WJ. EMBO J. 2002 Dec 16; 21(24):6721-32.
Functional consequences of single amino acid substitutions in calmodulin-activated adenylate cyclase of Bordetella pertussis. [EMBO J. 1991]
Functional consequences of single amino acid substitutions in calmodulin-activated adenylate cyclase of Bordetella pertussis.
Glaser P, Munier H, Gilles AM, Krin E, Porumb T, Bârzu O, Sarfati R, Pellecuer C, Danchin A. EMBO J. 1991 Jul; 10(7):1683-8.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Binding of 3'-anthraniloyl-2'-deoxy-ATP to calmodulin-activated adenylate cyclas...
Binding of 3'-anthraniloyl-2'-deoxy-ATP to calmodulin-activated adenylate cyclase from Bordetella pertussis and Bacillus anthracis.
J Biol Chem. 1990 Nov 5 ;265(31):18902-6.

PubMed
An unusual case of congenital melanocytic nevus presenting as neurocutaneous mel...
An unusual case of congenital melanocytic nevus presenting as neurocutaneous melanoma coexisting with Tuberous Sclerosis complex: A case report.
J Med Case Rep. 2011 Jul 1 ;5:267.

PubMed
Control of endogenous phosphorylation of the major cAMP-dependent protein kinase...
Control of endogenous phosphorylation of the major cAMP-dependent protein kinase substrate in adipocytes by insulin and beta-adrenergic stimulation.
J Biol Chem. 1990 Nov 5 ;265(31):18769-75.

PubMed
PRDM1/Blimp1 downregulates expression of germinal center genes LMO2 and HGAL.
PRDM1/Blimp1 downregulates expression of germinal center genes LMO2 and HGAL.
FEBS J. 2011 Sep ;278(17):3065-75. doi: 10.1111/j.1742-4658.2011.08227.x. Epub 2011 Aug 2 .

PubMed
Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and...
Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria.
J Bacteriol. 1990 Nov ;172(11):6568-72.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Binding of 3'-anthraniloyl-2'-deoxy-ATP to calmodulin-activated adenylate cyclase from Bordetella pertussis and Bacillus anthracis.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Cited by 3 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information