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J Am Soc Nephrol. 2011 Jul;22(7):1286-96. doi: 10.1681/ASN.2010080860. Epub 2011 Jun 30.

WT1-dependent sulfatase expression maintains the normal glomerular filtration barrier.

Author information

  • 1Department of Medicine, Children’s Hospital Boston and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA. Valerie.Schumacher@childrens.harvard.edu

Erratum in

  • J Am Soc Nephrol. 2011 Dec;22(12):2332. Pavenstaedt, Hermann [corrected to Pavenstädt, Hermann].

Abstract

Paracrine signaling between podocytes and glomerular endothelial cells through vascular endothelial growth factor A (VEGFA) maintains a functional glomerular filtration barrier. Heparan sulfate proteoglycans (HSPGs), located on the cell surface or in the extracellular matrix, bind signaling molecules such as VEGFA and affect their local concentrations, but whether modulation of these moieties promotes normal crosstalk between podocytes and endothelial cells is unknown. Here, we found that the transcription factor Wilms' Tumor 1 (WT1) modulates VEGFA and FGF2 signaling by increasing the expression of the 6-O-endosulfatases Sulf1 and Sulf2, which remodel the heparan sulfate 6-O-sulfation pattern in the extracellular matrix. Mice deficient in both Sulf1 and Sulf2 developed age-dependent proteinuria as a result of ultrastructural abnormalities in podocytes and endothelial cells, a phenotype similar to that observed in children with WT1 mutations and in Wt1(+/-) mice. These kidney defects associated with a decreased distribution of VEGFA in the glomerular basement membrane and on endothelial cells. Collectively, these data suggest that WT1-dependent sulfatase expression plays a critical role in maintaining the glomerular filtration barrier by modulating the bioavailability of growth factors, thereby promoting normal crosstalk between podocytes and endothelial cells.

Copyright © 2011 by the American Society of Nephrology

PMID:
21719793
[PubMed - indexed for MEDLINE]
PMCID:
PMC3137576
Free PMC Article
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