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    Nucleic Acids Res. 2011 Oct;39(18):7881-99. Epub 2011 Jun 28.

    Internal standard-based analysis of microarray data2--analysis of functional associations between HVE-genes.

    Source

    Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. igor-dozmorov@omrf.org

    Abstract

    In this work we apply the Internal Standard-based analytical approach that we described in an earlier communication and here we demonstrate experimental results on functional associations among the hypervariably-expressed genes (HVE-genes). Our working assumption was that those genetic components, which initiate the disease, involve HVE-genes for which the level of expression is undistinguishable among healthy individuals and individuals with pathology. We show that analysis of the functional associations of the HVE-genes is indeed suitable to revealing disease-specific differences. We show also that another possible exploit of HVE-genes for characterization of pathological alterations is by using multivariate classification methods. This in turn offers important clues on naturally occurring dynamic processes in the organism and is further used for dynamic discrimination of groups of compared samples. We conclude that our approach can uncover principally new collective differences that cannot be discerned by individual gene analysis.

    PMID:
    21715372
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC3185418
    Free PMC Article

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