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Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638.

A public genome-scale lentiviral expression library of human ORFs.

Author information

  • 1RNA interference (RNAi) Platform, Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Abstract

Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

PMID:
21706014
[PubMed - indexed for MEDLINE]
PMCID:
PMC3234135
Free PMC Article
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