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Appl Environ Microbiol. 2011 Aug;77(15):5566-8. doi: 10.1128/AEM.00632-11. Epub 2011 Jun 24.

Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009.

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  • 1Dept. of Environment and Agro-biotechnologies (EVA), Centre de Recherche Public- Gabriel Lippman, 41 Rue du Brill, L-4422 Belvaux, Luxembourg.


Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.

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