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Clin Biochem. 2011 Sep;44(13):1050-7. doi: 10.1016/j.clinbiochem.2011.06.004. Epub 2011 Jun 17.

Detection of circulating tumor cells in peripheral blood from patients with gastric cancer using piRNAs as markers.

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  • 1Ningbo University School of Medicine, Ningbo 315211, China.

Abstract

OBJECTIVE:

To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers.

DESIGN AND METHODS:

Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in the peripheral blood of 93 patients with gastric cancer and 32 healthy volunteers. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic values.

RESULTS:

The peripheral blood levels of piR-651 and piR-823 in the patients with gastric cancer were significantly lower than those from controls (P<0.001). The piR-651 level in gastric adenocarcinoma was higher than that in gastric signet ring cell carcinoma (P = 0.003). The piR-823 level was positively associated with tumor-node-metastasis stage (P = 0.027) and distant metastasis (P = 0.026). The areas under the ROC curve were 0.841, 0.812 and 0.860 for piR-651, piR-823 and the combination, respectively.

CONCLUSIONS:

piRNAs may be valuable biomarkers for detecting circulating gastric cancer cells.

Copyright © 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

PMID:
21704610
[PubMed - indexed for MEDLINE]
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