Science. 1990 Sep 21;249(4975):1423-5.

An insertion in the human thyrotropin receptor critical for high affinity hormone binding.

Wadsworth HL, Chazenbalk GD, Nagayama Y, Russo D, Rapoport B.

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Department of Medicine, Veterans Administration Medical Center, San Francisco, CA 94121.

Abstract

Thyrotropin (TSH), luteinizing hormone (LH), and chorionic gonadotropin (CG) are structurally related glycoprotein hormones, which bind to receptors that share a high degree of sequence similarity. However, comparison of the primary amino acid sequences of the TSH and LH-CG receptors reveals two unique insertions of 8 and 50 amino acids in the extracellular domain of the TSH receptor. The functional significance of these insertions were determined by site-directed mutagenesis. Deletion of the 50-amino acid tract (residues 317 to 366) had no effect on TSH binding or on TSH and thyroid-stimulating immunoglobulin (TSI) biological activities. In contrast, either deletion or substitution of the eight-amino acid region (residues 38 to 45) abolished these activities. This eight-amino acid tract near the amino terminus of the TSH receptor appears to be an important site of interaction for both TSH and TSI.

PMID:: 2169649; [PubMed - indexed for MEDLINE]

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Thyrotropin-luteinizing hormone/chorionic gonadotropin receptor extracellular domain chimeras as probes for thyrotropin receptor function. [Proc Natl Acad Sci U S A. 1991]
Thyrotropin-luteinizing hormone/chorionic gonadotropin receptor extracellular domain chimeras as probes for thyrotropin receptor function.
Nagayama Y, Wadsworth HL, Chazenbalk GD, Russo D, Seto P, Rapoport B. Proc Natl Acad Sci U S A. 1991 Feb 1; 88(3):902-5.
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Review The thyrotropin receptor 25 years after its discovery: new insight after its molecular cloning.
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An insertion in the human thyrotropin receptor critical for high affinity hormone binding.

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