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Nat Commun. 2011 Jun 21;2:359. doi: 10.1038/ncomms1367.

Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome.

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  • 1Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

Centrosomes are conserved organelles that are essential for accurate cell division and cilium formation. A centrosome consists of a pair of centrioles surrounded by a protein network of pericentriolar material (PCM) that is essential for the centrosome's function. In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole. When Sas-4 is absent, nascent procentrioles are unstable and lack PCM, and functional centrosomes are not generated. When Sas-4 is mutated, so that it cannot form S-CAP complexes, centrosomes are present but with dramatically reduced levels of PCM. Finally, purified S-CAP complexes or recombinant Sas-4 can bind centrosomes stripped of PCM, whereas recombinant CNN or Asl cannot. In summary, PCM assembly begins in the cytosol where Sas-4 provides a scaffold for pre-assembled cytoplasmic complexes before tethering of the complexes in a centrosome.

PMID:
21694707
[PubMed - indexed for MEDLINE]
PMCID:
PMC3677532
Free PMC Article
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