Proc Natl Acad Sci U S A. 1990 Sep;87(17):6922-6.

A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding.

Kaye FJ, Kratzke RA, Gerster JL, Horowitz JM.

Source

National Cancer Institute-Navy Medical Oncology Branch, Naval Hospital, Bethesda, MD 20814.

Abstract

We have previously identified a small-cell lung cancer cell line (NCI-H209) that expresses an aberrant, underphosphorylated form of the retinoblastoma protein RB1. Molecular analysis of RB1 mRNA from this cell line revealed a single point mutation within exon 21 that resulted in a nonconservative amino acid substitution (cysteine to phenylalanine) at codon 706. Stable expression of this mutant RB1 cDNA in a human cell line lacking endogenous RB1 demonstrated that this amino acid change was sufficient to inhibit phosphorylation. In addition, this cysteine-to-phenylalanine substitution also resulted in loss of RB1 binding to the simian virus 40 large tumor and adenovirus E1A transforming proteins. These results confirm the importance of exon 21 coding sequences and suggest that the cysteine residue at codon 706 may play a role in achieving a specific protein conformation essential for protein-protein interactions.

PMID:: 2168563; [PubMed - indexed for MEDLINE]
PMCID:: PMC54650

Free PMC Article

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Medical

RB1 Gene - Genetics Home Reference

Research Materials

Supplemental Content

Save items

Related citations in PubMed

Functional analysis at the Cys706 residue of the retinoblastoma protein. [J Biol Chem. 1992]
Functional analysis at the Cys706 residue of the retinoblastoma protein.
Kratzke RA, Otterson GA, Lin AY, Shimizu E, Alexandrova N, Zajac-Kaye M, Horowitz JM, Kaye FJ. J Biol Chem. 1992 Dec 25; 267(36):25998-6003.
The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. [Science. 1989]
The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product.
Dyson N, Howley PM, Münger K, Harlow E. Science. 1989 Feb 17; 243(4893):934-7.
Mutations in the RB1 gene and their effects on transcription. [Mol Cell Biol. 1989]
Mutations in the RB1 gene and their effects on transcription.
Dunn JM, Phillips RA, Zhu X, Becker A, Gallie BL. Mol Cell Biol. 1989 Nov; 9(11):4596-604.
Point mutational inactivation of the retinoblastoma antioncogene. [Science. 1989]
Point mutational inactivation of the retinoblastoma antioncogene.
Horowitz JM, Yandell DW, Park SH, Canning S, Whyte P, Buchkovich K, Harlow E, Weinberg RA, Dryja TP. Science. 1989 Feb 17; 243(4893):937-40.
Two COOH-terminal truncated cytoplasmic forms of topoisomerase II alpha in a VP-16-selected lung cancer cell line result from partial gene deletion and alternative splicing. [Biochemistry. 1997]
Two COOH-terminal truncated cytoplasmic forms of topoisomerase II alpha in a VP-16-selected lung cancer cell line result from partial gene deletion and alternative splicing.
Yu Q, Mirski SE, Sparks KE, Cole SP. Biochemistry. 1997 May 13; 36(19):5868-77.

See reviews... See all...

Cited by 46 PubMed Central articles

Identification and characterization of retinoblastoma gene mutations disturbing apoptosis in human breast cancers. [Mol Cancer. 2010]
Identification and characterization of retinoblastoma gene mutations disturbing apoptosis in human breast cancers.
Berge EO, Knappskog S, Geisler S, Staalesen V, Pacal M, Børresen-Dale AL, Puntervoll P, Lillehaug JR, Lønning PE. Mol Cancer. 2010 Jul 1; 9:173. Epub 2010 Jul 1.
A cancer derived mutation in the retinoblastoma gene with a distinct defect for LXCXE dependent interactions. [Cancer Cell Int. 2010]
A cancer derived mutation in the retinoblastoma gene with a distinct defect for LXCXE dependent interactions.
Henley SA, Francis SM, Demone J, Ainsworth P, Dick FA. Cancer Cell Int. 2010 Mar 18; 10:8. Epub 2010 Mar 18.
A small-cell lung cancer genome with complex signatures of tobacco exposure. [Nature. 2010]
A small-cell lung cancer genome with complex signatures of tobacco exposure.
Pleasance ED, Stephens PJ, O'Meara S, McBride DJ, Meynert A, Jones D, Lin ML, Beare D, Lau KW, Greenman C, et al. Nature. 2010 Jan 14; 463(7278):184-90. Epub 2009 Dec 16.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A single amino acid substitution results in a retinoblastoma protein defective i...
A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding.
Proc Natl Acad Sci U S A. 1990 Sep ;87(17):6922-6.

PubMed
Scorecards: a new method to prevent adverse drug events? Preliminary results fro...
Scorecards: a new method to prevent adverse drug events? Preliminary results from a clinical field study.
Stud Health Technol Inform. 2011 ;166:234-45.

PubMed
Probabilistic cost-effectiveness analysis of cascade screening for familial hype...
Probabilistic cost-effectiveness analysis of cascade screening for familial hypercholesterolaemia using alternative diagnostic and identification strategies.
Heart. 2011 Jul ;97(14):1175-81.

PubMed
Divergent RNA binding specificity of yeast Puf2p.
Divergent RNA binding specificity of yeast Puf2p.
RNA. 2011 Aug ;17(8):1479-88. Epub 2011 Jun 17 .

PubMed
Ticagrelor versus clopidogrel in patients with acute coronary syndromes intended...
Ticagrelor versus clopidogrel in patients with acute coronary syndromes intended for non-invasive management: substudy from prospective randomised PLATelet inhibition and patient Outcomes (PLATO) trial.
BMJ. 2011 Jun 17 ;342:d3527. doi: 10.1136/bmj.d3527.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding.

Source

Abstract

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

Research Materials

Supplemental Content

Save items

Related citations in PubMed

Cited by 46 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information