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Biochem Biophys Res Commun. 2011 Jul 8;410(3):574-80. doi: 10.1016/j.bbrc.2011.06.028. Epub 2011 Jun 12.

Sphingolipid long chain base phosphates can regulate apoptotic-like programmed cell death in plants.

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  • 1School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland.

Abstract

Sphingolipids are ubiquitous components of eukaryotic cells and sphingolipid metabolites, such as the long chain base phosphate (LCB-P), sphingosine 1 phosphate (S1P) and ceramide (Cer) are important regulators of apoptosis in animal cells. This study evaluated the role of LCB-Ps in regulating apoptotic-like programmed cell death (AL-PCD) in plant cells using commercially available S1P as a tool. Arabidopsis cell cultures were exposed to a diverse array of cell death-inducing treatments (including Cer) in the presence of S1P. Rates of AL-PCD and cell survival were recorded using vital stains and morphological markers of AL-PCD. Internal LCB-P levels were altered in suspension cultured cells using inhibitors of sphingosine kinase and changes in rates of death in response to heat stress were evaluated. S1P reduced AL-PCD and promoted cell survival in cells subjected to a range of stresses. Treatments with inhibitors of sphingosine kinase lowered the temperature which induced maximal AL-PCD in cell cultures. The data supports the existence of a sphingolipid rheostat involved in controlling cell fate in Arabidopsis cells and that sphingolipid regulation of cell death may be a shared feature of both animal apoptosis and plant AL-PCD.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
21683064
[PubMed - indexed for MEDLINE]
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