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Croat Med J. 2011 Jun;52(3):293-8.

New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks.

Author information

  • 1Institute of Forensic Science, Ministry of Public Security, Beijing, China.

Abstract

AIM:

To isolate mucosal cells of the perpetrator in a sexual assault case from a complex mixture of his mucosal cells and the victim's skin by micromanipulation prior to genomic analysis.

METHODS:

To capture and analyze mucosal cells we used the micromanipulation with on-chip low volume polymerase chain reaction (LV-PCR). Consensus DNA profiles were generated from 5 replicate experiments.

RESULTS AND CONCLUSIONS:

We validated the use of micromanipulation with on-chip LV-PCR for genomic analysis of complex biological mixtures in a fatal rape case. The perpetrator's mucosal cells were captured from nipple swabs of the victim, and a single-source DNA profile was generated from cell mixtures. These data suggest that micromanipulation with on-chip LV-PCR is an effective forensic tool for the analysis of specific cells from complex samples.

PMID:
21674825
[PubMed - indexed for MEDLINE]
PMCID:
PMC3118714
Free PMC Article

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