Frequency of CXCR4-using viruses in primary HIV-1 infections using ultra-deep pyrosequencing

AIDS. 2011 Aug 24;25(13):1668-70. doi: 10.1097/QAD.0b013e3283498305.

Abstract

We used ultra-deep pyrosequencing and the Toulouse Tropism Test phenotypic assay to determine the prevalence of CXCR4-using viruses in 21 patients with primary HIV-1 infections. We found X4-containing virus populations in 9% of patients by ultra-deep pyrosequencing using position-specific scoring matrices (PSSM(X4/R5)) or geno2pheno(5.75) and in 14% using the combined 11/25 and net charge rule. The phenotypic assay identified 9% of CXCR4-using viruses. This confirms that R5 viruses are predominant in primary HIV-1 infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • France
  • HIV Infections / virology*
  • HIV-1 / genetics*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Middle Aged
  • RNA, Viral
  • Receptors, CCR5 / genetics*
  • Receptors, CXCR4 / genetics*
  • Sequence Analysis, DNA / methods
  • Viral Proteins / genetics

Substances

  • RNA, Viral
  • Receptors, CCR5
  • Receptors, CXCR4
  • Viral Proteins