Abstract
A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta(®). Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.
MeSH terms
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Amino Acid Motifs
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Bacillus / enzymology
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Bacillus / genetics*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Cloning, Molecular
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DNA, Bacterial
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Escherichia coli
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Isoenzymes / genetics
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Isoenzymes / metabolism*
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Lactose / metabolism
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Plasmids
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism*
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Restriction Mapping
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Sequence Homology, Amino Acid
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Transformation, Bacterial
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism*
Substances
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Bacterial Proteins
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DNA, Bacterial
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Isoenzymes
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Recombinant Proteins
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beta-Galactosidase
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Lactose