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Anal Chem. 1999 Oct 1;71(19):4173-7. doi: 10.1021/ac990372u.

Microchip flow cytometry using electrokinetic focusing.

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  • 1Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, Tennessee 37831-6142.

Abstract

Flow cytometry of fluorescently labeled and unlabeled latex particles is demonstrated on a microfabricated device. The latex particles were detected and counted using laser light scattering and fluorescence coincidence measurements. Sample confinement was accomplished using electrokinetic focusing at a cross intersection, and detection occurred 50 μm downstream from the intersection. Particles with diameters of 1 and 2 μm were analyzed and distinguished from each other based on their light scattering intensity and fluorescence. A maximum sample throughput of 34 particles/s was achieved. Sample mixtures with varying proportions of fluorescently labeled and unlabeled particles were also analyzed and found to be within experimental error of the expected ratios.

PMID:
21662848
[PubMed]
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