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J Clin Microbiol. 2011 Aug;49(8):3044-6. doi: 10.1128/JCM.00486-11. Epub 2011 Jun 8.

Precision across the analytical measuring range of a quantitative real-time PCR assay for cytomegalovirus detection among three clinical laboratories.

Author information

  • 1Molecular Microbiology Laboratory, Mayo Medical Laboratories New England, 160 Dascomb Rd., Andover, MA 01810, USA. grys.thomas@mayo.edu

Abstract

This study measured the precision of a quantitative laboratory-developed real-time PCR test for cytomegalovirus performed at three different clinical laboratories that use the same methodology. The overall standard deviation (adjusted for analyte level) was 0.18 log(10) copies/ml, and there was no significant relationship between standard deviation and analytical measuring range.

PMID:
21653778
[PubMed - indexed for MEDLINE]
PMCID:
PMC3147729
Free PMC Article
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