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PLoS One. 2011;6(5):e19683. doi: 10.1371/journal.pone.0019683. Epub 2011 May 19.

Regulation of zebrafish hatching by tetraspanin cd63.

Author information

  • 1Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom. m.trikic@shef.ac.uk

Abstract

Tetraspanins cause the clustering of membrane proteins into a level of organisation essential for cellular function. Given the importance and complicated nature of this mechanism, we attempted a novel approach to identify the function of a single component in a biologically relevant context. A morpholino knockdown strategy was used to investigate the role of cd63, a membrane protein associated with intracellular transport and a melanoma marker, in embryonic zebrafish. By using three separate morpholinos targeting cd63, we were able to identify a specific phenotype. Strikingly, morphant fish failed to hatch due to the lack of secreted proteolytic enzymes required for chorion-softening. The morphology of the hatching gland at both the cellular and intracellular levels was disorganised, suggesting a role for cd63 in the functioning of this organ. This work identifies a specific role for cd63 in the zebrafish embryo and provides evidence for the suitability of zebrafish as a model system for the investigation of tetraspanin enriched microdomains.

PMID:
21625559
[PubMed - indexed for MEDLINE]
PMCID:
PMC3098263
Free PMC Article

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