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Cell Stem Cell. 2011 Jun 3;8(6):695-706. doi: 10.1016/j.stem.2011.04.002.

miR-371-3 expression predicts neural differentiation propensity in human pluripotent stem cells.

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  • 1Developmental Biology Program, Sloan-Kettering Institute, New York, NY 10065, USA.

Abstract

The use of pluripotent stem cells in regenerative medicine and disease modeling is complicated by the variation in differentiation properties between lines. In this study, we characterized 13 human embryonic stem cell (hESC) and 26 human induced pluripotent stem cell (hiPSC) lines to identify markers that predict neural differentiation behavior. At a general level, markers previously known to distinguish mouse ESCs from epiblast stem cells (EPI-SCs) correlated with neural differentiation behavior. More specifically, quantitative analysis of miR-371-3 expression prospectively identified hESC and hiPSC lines with differential neurogenic differentiation propensity and in vivo dopamine neuron engraftment potential. Transient KLF4 transduction increased miR-371-3 expression and altered neurogenic behavior and pluripotency marker expression. Conversely, suppression of miR-371-3 expression in KLF4-transduced cells rescued neural differentiation propensity. miR-371-3 expression level therefore appears to have both a predictive and a functional role in determining human pluripotent stem cell neurogenic differentiation behavior.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
21624813
[PubMed - indexed for MEDLINE]
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