Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Jun;28(6):799-806. doi: 10.1080/19440049.2010.521959.

Determination of perchlorate in infant formula by isotope dilution ion chromatography/tandem mass spectrometry.

Author information

  • 1Food Research Division, Banting Research Center 2203D, Health Canada, Ottawa, Ontario, Canada.

Abstract

A sensitive and selective isotope dilution ion chromatography/tandem mass spectrometry (ID IC-MS/MS) method was developed and validated for the determination of perchlorate in infant formula. The perchlorate was extracted from infant formula by using 20 ml of methanol and 5 ml of 1% acetic acid. All samples were spiked with (18)O(4) isotope-labelled perchlorate internal standard prior to extraction. After purification on a graphitised carbon solid-phase extraction column, the extracts were injected into an ion chromatography system equipped with an Ionpac AS20 column for separation of perchlorate from other anions. The presence of perchlorate in samples was quantified by isotope dilution mass spectrometry. Analysis of both perchlorate and its isotope-labelled internal standard was carried out on a Waters Quattro Ultima triple quadrupole mass spectrometer operating in a multiple reaction monitoring (MRM) negative ionisation mode. The method was validated for linearity and range, accuracy, precision, sensitivity, and matrix effects. The limit of quantification (LOQ) was 0.4 µg l(-1) for liquid infant formula and 0.95 µg kg(-1) for powdered infant formula. The recovery ranged from 94% to 110% with an average of 98%. This method was used to analyse 39 infant formula, and perchlorate concentrations ranging from <LOQ to 13.5 µg l(-1).

PMID:
21623505
[PubMed - indexed for MEDLINE]
PMCID:
PMC3118528
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Atypon Icon for PubMed Central
    Loading ...
    Write to the Help Desk