Cell Stem Cell. 2011 Jun 3;8(6):633-8. doi: 10.1016/j.stem.2011.05.001.

Reprogramming of mouse and human cells to pluripotency using mature microRNAs.

Miyoshi N, Ishii H, Nagano H, Haraguchi N, Dewi DL, Kano Y, Nishikawa S, Tanemura M, Mimori K, Tanaka F, Saito T, Nishimura J, Takemasa I, Mizushima T, Ikeda M, Yamamoto H, Sekimoto M, Doki Y, Mori M.

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Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Yamadaoka 2-2, Osaka 565-0871, Japan.

Abstract

Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.

Comment in

Small RNAs loom large during reprogramming. [Cell Stem Cell. 2011]
Small RNAs loom large during reprogramming.Sridharan R, Plath K. Cell Stem Cell. 2011 Jun 3; 8(6):599-601.

PMID:: 21620789; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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GEO/GSE28586

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Reprogramming of mouse and human cells to pluripotency using mature microRNAs.
Reprogramming of mouse and human cells to pluripotency using mature microRNAs.
Cell Stem Cell. 2011 Jun 3 ;8(6):633-8. doi: 10.1016/j.stem.2011.05.001.

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