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Int J Mol Sci. 2010;11(12):5040-55. doi: 10.3390/ijms11125040. Epub 2010 Dec 7.

Matrix-assisted laser desorption/ionization imaging mass spectrometry.

Author information

  • 1Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; E-Mails: thaya@hama-med.ac.jp (T.H.); naoko.goto.inoue@gmail.com (N.G.-I.); setou@hama-med.ac.jp (M.S.).

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

KEYWORDS:

biomarker; imaging mass spectrometry; lipids; matrix-assisted laser desorption/ionization mass spectrometry; protein

PMID:
21614190
[PubMed - in process]
PMCID:
PMC3100838
Free PMC Article
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