Abstract
The interaction between dendritic cells (DCs) and natural killer (NK) cells plays a key role in inducing DC maturation for subsequent T-cell priming. We investigated to generate potent DCs by stimulated with NK cells to induce myeloma-specific cytotoxic T lymphocytes (CTLs). NK cells-stimulated-DCs exhibited high expression of costimulatory molecules and high production of IL-12p70. These DCs induce high potency of Th1 polarization and exhibit a high ability to generate myeloma-specific CTLs responses. These results suggest that functionally potent DCs can be generated by stimulation with NK cells and may provide an effective source of DC-based immunotherapy in multiple myeloma.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Polarity / drug effects
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Cell Polarity / immunology
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Cell Proliferation / drug effects
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Cells, Cultured
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Cytotoxicity, Immunologic / drug effects
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Cytotoxicity, Immunologic / physiology
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Dendritic Cells / drug effects
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Dendritic Cells / immunology*
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Dendritic Cells / pathology
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HL-60 Cells
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Humans
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Interferon Type I / pharmacology
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K562 Cells
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Killer Cells, Natural / drug effects
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Killer Cells, Natural / immunology
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Killer Cells, Natural / pathology
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Killer Cells, Natural / physiology*
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Lymphocyte Activation / drug effects
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Lymphocyte Activation / immunology
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Lymphocyte Activation / physiology*
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Multiple Myeloma / immunology*
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Multiple Myeloma / pathology
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Polydeoxyribonucleotides / pharmacology
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Recombinant Proteins
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T-Cell Antigen Receptor Specificity / drug effects
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T-Cell Antigen Receptor Specificity / immunology
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T-Lymphocytes, Cytotoxic / drug effects
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Cytotoxic / pathology
Substances
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Interferon Type I
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Polydeoxyribonucleotides
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Recombinant Proteins
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poly d(I-C)